- Schedule consultation with Platform Head and staff to discuss project.
- Sign and return platform acknowledgment and account form.
- Schedule directly with staff for equipment training. We support both screening and non-screening projects.
- After staff approval, users can sign up for independent equipment usage.
- Access to our libraries requires approval of a screening application after assay optimization and validation has been completed by the applicant.
- Test and pilot screens are performed by the user. If deemed successful, the user may continue with the rest of the assay plates.
Workflow & Built-In Quality Control at CHiP-GIS
If the test plate or pilot screen showed unsatisfactory results, the screener must return to his/her home institution for further assay optimization. If a screen is not performed, the screener will be charged for instrumentation usage.
Information for formatting and uploading data will be provided after your pilot screen is done. Once data is entered, directions will be provided for viewing data and submit wells of interest and hits.
Sample (eukaryotic cells) submission guidelines
- HTS applications require about 15-20% more sample volume during cell seeding compared to manual seeding; the automated liquid dispenser uses dead volume during priming and in between plates.
Keeping this in mind, the screener must submit adequate sample volume for cell seeding.
- Screener must optimize sample for cell health and viability in HTS plates for culture conditions (media, cell numbers, in HTS plates, and duration of cell incubation) before submission.
- Screener must use precaution to grow samples in a contaminants-free environment and to inspect the sample for possible contamination before submission.
- If screener is supplying the assay reagent, please supply adequate (15% more) reagent for the experiment.